In many practical applications the space requirements are the main obstacle for using a dynamic programming algorithm.
However, the greatest obstacle for using custom arrays is the lack of functional annotation for ESTs.
That is the main obstacle for using conductive copper ink because the appearance of copper oxide will result in decreasing the electrical conductivity.
However, the main obstacle for using this technique on large-scale assessment data is the difficulty in finding proper IVs.
The main obstacle for using this method is absence of commercial preparations of primary amine oxidase (AMO; E.C. 1.4.3.21), the key biologically active element of a potential biosensor.
As with other cancer-specific promoters, the low promoter activity is a major obstacle for using CEA promoter-based prodrug gene therapy in vivo and in clinical trials.
One of the main obstacles for using the power-shaping approach is to write the dynamics in the required form, since a partial differential equation system submitted to sign constraints has to be solved.
Another obstacle for using polymer-coated magnetic particles for gene delivery is the inability of the MNPs to express effectively at the target site.
One obstacle for using one stimulus for both DC subsets is that they do not express an overlapping repertoire of TLRs [ 13].
Therefore, this minimal level of diversity seen in the 16S rRNA of B. anthracis, B. cereus, and B. thuringiensis was thought to be an obstacle for using 16S rRNA gene sequencing to identify and differentiate these three species.
