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We hypothesize that when the complementary single-stranded DNA attached to the Au forms the more stable double strand, the Au reorganizes on the microgel surface, yielding the observed unique Janus particle structures.
Additional studies are required to investigate the precise molecular mechanisms responsible for the observed, unique roles of the substitutions at position E2-60 and E2-211 of CHIKV.
Nonetheless, we restricted our comparisons to diets of adult snails and so this factor seems unlikely to be responsible for the observed unique diets of C. ebraeus at Hawaii and the Seychelles.
Further, because of the observed unique respiratory involvement of the acute NiV infection in the monkeys, we sought approval for bringing in a portable X-ray device into the BSL-4 and subsequently carried out a series of X-ray images on one animal with signs of acute NiV disease (Subject 3; see Table 1).
We suspect some of the observed unique differences in copy number aberration calling to be of biological importance.
The PCR products were resolved on 1.0% agarose gels, and DNA was extracted from the observed unique bands using the QIAquick Gel Extraction Kit (QIAGEN, Hilden, Germany).
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The number of observed unique ChIP-seq DNA fragments, M2, representing these BSs (N2), equals 299830; GDP estimates 297493 ChIP-seq DNA fragments.
In the observed dataset, unique indels were located closer to coding sequences than approximate indels (6,877 vs. 8,886 bp on average, p < 0.0001; Table S2A (see Additional file 1)).
Locus 2 has an observed unique tag 482 bases downstream of the stop codon (Index 3, Figure 3; mean cpm in MA glands = 6086).
cRichness is based on observed unique OTUs.
Figure 2 contains a complete list of all observed unique operons in our dataset.
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