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A logical next step is to identify the selective pressure(s) responsible for the observed selection patterns.
The only two polymorphisms in the middle portion of ZmGW2-CHR4 are in complete LD; thus, only two haplotypes were observed (Table 3; Figure 3), consistent with the observed selection and reduced nucleotide diversity in this region.
In order to compare the observed selection intensity with the effect of genetic drift we used two different estimates of the effective population size.
As a result, the observed selection for translational efficiency of highly expressed genes within a given genome must be constrained by the established parameters of that genome as suggested by the analyses of Chen et al. [13].
Especially disconcerting is the observed selection with respect to the differentiation type.
Such mixing is expected to reduce the observed selection pressure arising from drug-treatment.
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Firstly, the deepest selection valley from each of 500 simulations (Additional File 3) of the experimental design were used to estimate the probability (null hypothesis) that the depth of an observed selection valley was the result of random processes working in combination with selection at minor loci for resistance (p-value for no major gene = 0.026, Figure 3A, Additional Files 4, 5).
Models based on either LWD or stream slope were equally likely without over-parameterization (ΔAICC ≤ 2.3) and explained between 56% and 70% of the variation in observed selection differentials.
But if the observed positive selection was due to selection for an optimized hydrolytic activity in the new host, we would expect to find relatively few β-xylosidase haplotypes among the domesticated M. graminicola strains as a result of purifying selection during the 10,000 years since wheat domestication.
Studies comparing cross type selection patterns in experimental crop wild hybrid zones with and without certain cross type(s) provide a way to identify whether certain cross types are the causative selective pressure(s) responsible for observed selection patterns.
For the F1 cross type, our observed selection gradient of 0.87 for early season leaf length is sufficient to invoke 'rapid microevolutionary changes' (Kingsolver et al. 2012; pp. 3), assuming moderate trait heritability (Falconer 1981).
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