Although this experimental system is thus not ideal to analyze the precise role of Poc1 at the centrosome, the observed cell division defects are entirely consistent with the various phenotypes documented previously following interference with Poc1 protein levels in a range of other species and cells.
Because both ptCpn60 and CRL [ 19] function in chloroplasts and, in our screening, several mutant plants that showed abnormal morphology contained chloroplasts of normal size, it is unlikely that the developmental defects are the cause of the observed chloroplast division defects.
These data allowed us to assess the evolutionary history (demography, age and origins) of various Chinese populations and to address the hypothesis that the observed phylogeographic divisions are consistent with past range fragmentation by Pleistocene glaciations.
Since MMC stops cell division, the observed decrease in cell number in the presence of MMC suggested cell division must have occurred at the same locations in untreated samples.
The observed kinetics of cell division and cell accumulation was similar to that seen with peptide-stimulated non-tolerant control T cells of Agneg mice.
The proposed role of ATAD3 as a regulator of nucleoid formation and/or segregation is in accordance with the observed alterations in cell division after knock-down of ATAD3 [9], [9] and receives additional importance with regard to high protein expression levels in certain types of cancer.
The observed defects in plastid division in a series of ptcpn60β mutants even in the presence of wild type ptCpn60β alleles may reflect disruption of the stoichiometric relationship of functional plastid division proteins due to misfolding in the mutants after import from the cytosol.
Thus the full machinery developed in MT, e.g. division of the observed field into normal and anomalous parts, is not necessarily needed in GIC studies.
We therefore attempt to bring the model closer to the observed biology by formulating cell division as an asynchronous process, using the formulation of stochastic Markov processes, in which time is now a continuous, rather than a discrete, quantity (see Materials and Methods for details).
