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At the beginning (Figure 3a), the great nuclear area is elevated from the extended cellular membrane; the non-nucleus part of the great nuclear area (green triangles) is higher than the nucleus part (yellow triangles); the whole cell is flat but the surface is uneven and wavy (Figure 3a-d).
Myosin Va is mainly distributed within the nucleus, part of which is associated with the evaginated nuclear membrane during AT formation.
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We discovered that at the beginning of the late spermatid stage, when the nucleus initiates to protrude at the bottom of the nuclear cup, accompanied by acrosome invagination, myosin Va allocates within the evaginated nucleus part and MC (Figure 6E, F).
The proposed scheme has two parts: The nucleus segmentation part is based on morphological analysis, and the cytoplasm segmentation is based on pixel-intensity thresholding.
Taking into account that RPB3 protein exerts its crucial role in the nucleus as part of the pol II core enzyme, we demonstrated that eEF1γ co-immunoprecipitates with the pol II enzyme.
Yellow triangles: nucleus part of the great nuclear area.
This nucleus, part of the interoceptive lamina I pathway, projects to cingulate and prefrontal cortex.
Then the non-nucleus part of the great nuclear area gradually merges to the nucleus (Figure 3a-f), and cannot be distinguished from the nucleus 30 min later (Figure 3f).
(Previous work has shown that when women who had never had babies were shown manipulated super cute pictures of babies, they had increased activity in the nucleus accumbens a part of the brain important for the reward system).
Phototransfection with Elk-1 GFP mRNA shows that Elk-1 translocates from the dendrite into the nucleus as part of the cell death process.
We also tested the effects of RbBP5 knockdown since it is known to to form a complex with mDpy-30 in the nucleus as part of the H3K4MT.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com