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Before normalization, the normal distribution and linear relations of Cy3 and Cy5 intensities were tested by qqplot and a linear regression model, respectively, in R statistical language.
We later derived the P-value of each test from the normal distribution after normalization.
Such 'structural distribution' reflected therefore the anisotropy of the structure due to the finite scale of the computations, and we used it as a normalization distribution.
After each of the normalizations, the distribution bias and the cluster of Chip22 were no longer present, suggesting the minor batch effects could be corrected by simple normalization.
The natural log-transformed lead was used to assist in the normalization of the distribution for blood Pb levels, since the distribution of Pb levels on the original scale was right-skewed.
However, the normalization condition for distribution function (4) (5).
After the normalization, the bias distribution was removed in both control and RDX treated samples.
We found that the removal of low-expressed genes after the normalization and data distribution fitting procedures was the most flexible filtering strategy.
HA injections may stimulate endogenous production of additional HA by human synoviocytes, aiding in the normalization of HA distribution within the synovial fluid [ 124].
This approach allows for the "normalization" of the distribution of voxel-to-voxel connectivity values as effectively as would inclusion of the global signal as a covariate of no interest, but without the potential problems of the latter method.
The normalization of the distribution also made it possible to improve the statistical validity of comparisons and to obtain better results in satisfaction score modelling when adjusted variables are tested.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com