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The normalised ratios were log10-transformed before statistical analysis.
Finally, the normalised ratios were calculated.
The normalised ratios are the computed ratios minus the expected values on the curve.
Data analysis was based on the normalised ratios of Cy5/Cy3 signals observed for each BAC clone that previously passed the flag assessment procedure.
The normalised ratios can be used as an index of the relative intensity of a given peak in a given sample, and hence be used to detect copy number changes.
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In all three clones studied, the normalised ratio (total signal Probe 1)/ total signal Probe dhps) deviated considerably from 1, ranging from 1.8 (clone C2) to 2.5 (clone E10, Fig. 3B).
The normalised ratio (Rnorm), proportional to the Ca2+ concentration, was calculated as described previously [30].
The accuracy of measurement by each probe was evaluated by calculating the standard deviation (SD) of the normalised ratio.
The normalised ratio of BAC 548I13 is at zero, even though it has three copies, as NCI-H526 has greater than a diploid number of chromosomes.
The normalised ratio of each target on MYH9 and Rb was expected to be close to 1, if no deletion had occurred.
The normalised ratio of each target on 8q11 SST was expected to be close to 1 if no deletion was present.
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