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Step 6 e) assigns the first | R t, h ′ ∪ B | − v h C orders from R t, h ′ ∪ B to B and proceed to the next secondary iteration.
Based on the normed and academic T-scores in the PSLE, all students will be sorted and allocated to different academic tracks (such as 4 years' integrated programme, 5 years secondary schools or vocational schools) in the next secondary level of schooling system (Koh and Luke, 2009; Luke, Freebody et al., 2005).
The slides were blocked again with the avidin biotin kit and were incubated with the next secondary biotinylated horse anti-mouse antibody (IgG heavy and light chain; Vector Laboratories), followed by streptavidin-conjugated fluorochrome Alexa 546 (Molecular Probes).
The next secondary analysis was completed in a limited cohort (n = 462) to confirm any association found based on residential history using mean speciated urinary arsenic concentrations (As3+, As5+, MMA, and DMA).
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Upon the modification at the first stage with a small (relative to other stages) amount of long-chain oligomers, an interaction with particles is realized on the external agglomerates surface with formation at the next stages secondary polymeric layer rendered over the surface (Figs. 1 and 5).
The next day, secondary antibody was applied and the signal was visualized on a Molecular Imager ChemidocXRS (Bio-Rad Laboratheies) using the Pierce Supersignal West Pico chemiluminescent substrate (Thermo Scientific).
The next day, secondary antibodies were incubated for 1 h at room temperature and visualized using enhanced chemiluminescence (Millipore, MA, USA).
The next day, secondary biotinylated horse anti-mouse IgG (1 200, Vector Laboratories, Burlingame, CA, USA) was applied to the slides for 1 h at room temperature VECTASTAIN Elite ABC kit (Vector Laboratories) and 3, 3′-diaminobenzidine (DAB; Sigma-Aldrich) were subsequently used to reveal BrdU immunoreactivity.
Zinc-air batteries have a great potential to be the next generation of secondary batteries.
The next day, the secondary antibodies (goat anti-rabbit IgG, or donkey anti-goat IgG, (Table 1)) were applied for 2 hours.
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