Sentence examples for the next prepared from inspiring English sources

Lambert's TV talent-show training – a Michael Jackson cover one week, AC/DC the next – prepared him for life in a band whose output ran from metal to vaudeville.

To verify the importance of the aryl group, the substrate 43 was next prepared (Scheme 3).

From these solutions, were next prepared the solutions with about the concentrations of active substances (acetaminophen) equal to 0.30 mg · mL−1, 0.20 mg · mL−1, and 0.10 mg · mL−1.

We next prepared primary cultured fibroblasts from the biopsies of healthy donors and SCD-EDS patients who expressed the ZIP13G64D protein and compared the ZIP13 protein levels.

We next prepared versions with tandem repeats of the red acceptor FPs.

We next prepared cell lysates from 293T cells in the presence or absence of phosphatase inhibitors, immunoprecipitated the endogenous TFIIB, and then immunoblotted with either the pS65-TFIIB antibody or general anti-TFIIB antibody .

We next prepared cultured Müller cells and RGCs, and further confirmed the specificity of cell type-specific markers used and the purity of our cultured cells.

We next prepared membrane fractions from cells expressing either WT Hhat or the truncation mutants, and analyzed PAT activity in an in vitro Shh palmitoylation assay.

Therefore, we next prepared co-transfection/immunoprecipitation experiments using truncated recombinant SUN and KASH protein constructs co-expressed in the mammalian HeLa cell culture system referring to the approaches described by Stewart-Hutchinson et al. [5].

To examine if impaired binding of Sp1 and Sp3 to these sites impacted transcriptional regulation, we next prepared luciferase reporters matching sequences from −200 to +87 from Pongo, Hylobates moloch, and Nomascus l. leucogenys.

We next prepared sensory neuron/fibroblast co-cultures for TEM using fixation and staining procedures optimized to visualize extracellular proteins (see section Materials and methods).