Sentence examples for the ncre from inspiring English sources

In addition, a linker sequence (amino acid sequence ASPSNPGASNGS) [15] was added to connect the ERT2-domain and the NCre- or CCre part of the fusion protein.

To generate pNCre-ERT2 and pCCre-ERT2-constructs, the Stop codon within pCMV-Tag2B-NCre- and pCMV-Tag3B-CCre-plasmids was removed and a SpeI restriction site was introduced at the 3'-end of the NCre- or CCre open reading frame.

This suggests that the ERT2 domain on the C-terminus of CCre might spatially interfere with the GCN4 protein-protein interaction domain of the NCre constructs or of CCre-ERT2 itself, as this interaction domain is not present in CreERT2 [13].

Transgenic animals were crossed to ROSA26-LoxP-STOP-LoxP-EYFP-reporter mice (ROSY; [17]) and recombination was analysed in triple-transgenic animals containing the NCre-, CCre- as well as the reporter allele.

We refer to these constructs below using the abbreviations NE, CE, EN and EC, respectively (the original NCre and CCre [15] will be referred to as N and C, respectively; Fig. 1).

For that purpose, we analysed the Cre-complementation of the GFAP-NCre and PLP-CCre transgenes in ROSY reporter mice (Fig. 2).

Since abundant recombination in oligodendrocytes and neurons could be detected in the PLP-NCre x PLP-CCre- and the PLP-Cre mice with the same ROSY reporter allele, we can think it highly unlikely that these cell types might have been targeted in GFAP-NCre x PLP-CCre mice, but were not observed due to poor expression of the ROSY reporter allele.

The ERT2-domain [13] was fused to the N-terminus or the C-terminus of the constitutively active NCre- and CCre-proteins [15].

The slightly reduced number of recombined cells in PLP-NCre x PLP-CCre mice compared to the maximal recombination achievable in PLP-Cre mice (Fig. S3, S4) can be attributed to not completely overlapping expression patterns of the PLP-NCre and PLP-CCre transgenes in the respective mouse lines.

To evaluate the potency of split-Cre, we analyzed the progeny of GFAP+/PLP+ cells during brain development using the GFAP-NCre x PLP-CCre-mice.

The plasmid pTR/NCre) enables the liver-specific expression of NCre (i.e., the Cre gene and the nuclear location signal) [ 15], the transcription of which is controlled by a liver-specific mouse transthyretin (i.e., prealbumin) promoter [ 16, 17].