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As automatic parameter estimation performed very poorly on non-exhaustive module detection methods (which include some clustering methods, see Supplementary Note 2), we assigned every unassigned gene to the module with which the average correlation was the highest prior to calculating the cluster validity indices.
Where components were not disjoint (i.e. were connected to multiple modules), the component was manually assigned to the module with which the majority of nodes were connected.
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In the alluvial diagram described in section 4, we want to associate each nonsignificant module with the module together with which it most likely forms a subset.
Then, starting at the smallest module, we associate the module with the other larger module with which it is most often clustered, and proceed to the next smallest module, and so on.
When characterizing the Mediator and RNAPII interaction in vitro a couple of years ago, RNAPII was typically visualized as binding to the Mediator through the previously defined 'Tail' module, which was far away from the formerly identified 'Head' module with which RNAPII should interact.
This process has been suggested to take place at the plasma membrane, possibly through a phosphoinositide interaction module with which BtpA inserts itself in the membrane [ 23- 25].
In fact, most PET scanners are hybrid devices that include a CT module, with which a CT for attenuation correction can be acquired.
These terms corresponds very well with the positive correlation of the Module with Gal and Ara content, which are typically found associated with hemicelluloses in grass species.
We find it convenient to summarize the gene expression profiles of a given module with the module eigengene, which can be considered as the best summary of the standardized module expression data [ 33, 41].
They are also negatively correlated with the module eigengene, which is consistent with higher expression in the less vascularised region of the inguinal fat pad, suggesting an inverse relationship between vascularisation and adiposity.
Having defined a set of modules with which to distinguish between global and local differences we were then able to quantify the degree of β-diversity for each module in each subtype and perform all pair-wise comparisons between the five intrinsic subtypes and normal breast tissue We performed a total of 30 pair-wise comparisons per module across all stages and subtypes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com