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Comparison of shoot responsive proteins between the three treatments revealed that the 46 significant modifications encompassed 23 distinct spots by reason of overlapping changes.
Approximately 4 million cells were prepared and assessed as previously described using a FLUOstar microplate reader (BMG Labtech Offenburg, Germany) (Alasmari et al., 2013a) with minor modifications encompassing use of 0.05% Pluronic acid F127 and manganese chloride (9.1 mM).
Methods for intein-mediated C-terminal protein modification encompass expressed protein ligation (EPL) and protein trans-splicing (PTS).
A modification encompassing both systems was described by Tauber et al, 1 who proposed that counting the number of, and percentage horizontal obliteration of the lower fornix by symblephara could potentially improve detection of disease progression.
There are three types of modification, that is, (i) broad regional modification encompassing gene body and often multiple genes, (ii) promoter‐specific peaks and (iii) X chromosome inactivation in females.
The fact that epigenetic modifications can encompass large regions of genomic DNA and that epigenetic marks display certain plasticity further supports this hypothesis [ 19].
The modifications include small changes.
The modifications cost $85,000.
The modifications, he said, were "hauntingly similar".
The modifications had various effects.
Moreover, the "modifications" are all rather radical.
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