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The mixture was split into two 200-µl flat-cap transparent tubes.
The beads were washed 3 times with 1% NT-2 buffer (1% Nonidet P-40, 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, and 2 mM EDTA) and the mixture was split in half.
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The mixture is split into two tubes and the probe is precipitated by the addition of 95% ethanol (800 μl) to each tube.
The mixtures were split into two fractions and thermolysin activity was inhibited in both the fractions with 0.4 mM (final concentration) phosphoramidon (Sigma-Aldrich, St . Louis MO).
After 5 min of preincubation, the reaction mixture was split in two.
Following this incubation, the reaction mixture was split into two halves and to one half a 10-fold excess of untagged duplex DNA (duplex 17) was added.
The reaction mixture was split into three aliquots.
The no-digest control mixture was split and subjected to two ddPCR duplexed assays of RASSF1/ RNaseP and RASSF1/ β-actin.
The mixtures were incubated for 37 °C for 2 h, 60 °C for 2 h, then 65 °C for 20 min. The restriction enzyme digested mixture was split and subjected to three ddPCR duplexed assays of SRY/ TERT, RASSF1/ RNaseP, and RASSF1/ β-actin.
The 200 μl reaction mixture was split into 4 tubes (50 μl each); PCR conditions were 2 min at 95°C, fourteen cycles of 10 s at 95°C, 30 s at 52°C, and 120 s at 72°C followed by a final extension for 5 min at 72°C.
In the first subunit the feed mixture is split into two fractions containing either a single component or a binary mixture.
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