Your English writing platform
Discover LudwigExact(35)
Since 100 RPKE was determined as the minimum expression for gene detection, Tiling Assembly identified 20,230 expressed annotated genes in rice aleurone.
This search returned a single hit, which appears to be a fragment of an ICK protein that was expressed exclusively in the venom gland, but did not meet the minimum expression level (>1 eCPM) for inclusion in the venom gland specific set.
(a) Reporter gene expression measured in luminescence/colony-forming units (CFU), relative to the minimum expression (mean±s.e.m., n=5).
Figure 2C shows that, at constant Fd/Fa of 2.0/1.5, the Sensitivity of detection of differential expression drops as the restriction for the minimum expression level (Em) decreases.
In order to test this hypothesis, for each family with k TFs (TF1, TF2, ‥, TFk) and each tissue T, we computed the ratio R = (Emax-Esim)/(Emax-Emin), where Emax and Emin represent the maximum and the minimum expression levels, respectively, among the family members in tissue T. Thus, the R value is normalized for family-specific and tissue-specific gene expression.
For each family of TF (F1, F2, ‥, Fk) and each tissue T, we computed the ratio RI = (Emax-Emax2)/(Emax-Emin), where Emax and Emin represent the maximum and the minimum expression levels and Emax2 represents the second highest expression among the family members in tissue T. The ratio RI captures the increase in expression, i.e., high value of RG represents an expression increase.
Similar(25)
The amplitude of regulation (the difference between the maximum and minimum expression values across the cell cycle) was calculated for all genes and was found to be independent of sequencing coverage for genes with more than ∼300 reads (Fig. 2B).
Briefly this involved calculating the MFC (maximal fold change), i.e. the ratio of the maximum and minimum expression values for all microarrays performed in the experiment, and the CV, i.e. the coefficient of variation.
For samples that showed no expression of the test gene, the value of minimum expression was used for statistical analysis.
First, identification of exons was achieved through analysis of overlapping reads aligning to the same region of the genome, with minimum expression of 50 RPKE required for these regions to be considered an exon.
It was designed to calculate the maximum and minimum expression values of putative ORFs in the growth (Max_L and Min_L), starvation (Max_S and Min_S) and conjugation (Max_C and Min_C) samples respectively.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com