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FISH followed essentially the methods already described in [ 16].
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The overall image data processing is based on the method already described elsewhere [12].
This method was based on the method already described to study active efflux in Salmonella enterica strains [40].
The file was then linked to the encrypted CHI dataset using the probability methods already described.
Given that the contents of the present manuscript have employed methods already described in a previous article (Brouillet et al., Biology Direct 2010, 5 40 doi:10.1186/1745-6150-5-40), although with an expanded range of application, I would have appreciated experimental data testing the critical assumptions of the analysis.
Profiles of M2 were estimated from PMU at vertical incidence at a vertical sampling of 20 m using the theoretical derivations and practical methods already described by Luce et al. (2017).
In order to evaluate the kinetics of murine anti-BMP-2 mAb release from each scaffold, 25 μg/mL of mAb was immobilized on each scaffold (titanium microbeads, alginate hydrogel, and ACS) according to methods already described in the literature [ 17].
The final validated UK English version of the survey was translated into Danish, Norwegian and Swedish, following methods already described [ 23].
Affymetrix oligonucleotide array hybridization was performed following methods already described (Neafsey et al. 2010), as previous analysis revealed a very high correlation between aggregate individual hybridizations and pooled hybridizations for pools of 20 individuals (Pearson's r = 0.96, Supporting Information, Figure S1), leading to the repetition of that assay design here.
The results show a better QOL for the BCS group than for the MRM accordingly to the scoring method already described.
Daily energy expenditure (DEE), fat mass (FM), fat-free mass (FFM) and water turnover were estimated over a period of 3 days by the Doubly Labeled Water (DLW) method already described [25], [26].
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