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Also, the RSD of the method was achieved to be 2.1%.
The study and optimization of the method was achieved through experimental design where recovery of compounds using acetonitrile for partition ranged from 62 to 99%.
The optimization of the method was achieved with a 27 4 Plackett Burman design, while the significant factors were optimized using a central composite design (CCD).
The robustness for the method was achieved through small modifications in our developed method whereby a low value for DOX (0.42 4.44%) in terms of %CV was observed, as shown in Table 2a.
Robustness of the method was achieved by introducing small changes in the compositions of mobile phase and detection wavelength.
Since the practical implementation of the method was achieved using a commercial-grade camera, the method and apparatus may be economically implemented in clinical practice.
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Adopting this alternative strategy the validation of several substances with significantly different permitted limits is considerably simplified and a deeper knowledge of the method is achieved.
We did not use ASTM method D2492 for sulfide determination because the determination of the sulfide form of sulfur in the method is achieved by extraction with dilute nitric acid, followed by determination of the iron content of the extract.
Evaluation of the methods was achieved through analysis of standard reference material, Oyster Tissue (NIST 1566b) and Tea (NCS DC 73351).
In this study, a 96.6%% of total coincidence rate and a substantial almost perfect agreement (k = 0.760) for genotyping 18 HPV types, which are capable detected in both the methods, was achieved between the BMRT HPV PCR assay and the HPV GenoArray test.
The validation of this method was achieved by comparison with previous reports applied to the same data set.
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