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The method required two sequential polymerase chain reaction stages, a primary and secondary assay.
To avoid silicate interferences, the method required an appropriate ratio of protons over molybdates equal to 70.
The method required acetylation of the nitrogen on the indole ring and used a palladium catalyst with copper II) acetate, 3-nitropyridine, and cesium pivalate.
It is shown that the method required to deal with these two cases are sufficient to deal also with systems having many zeros and poles.
The method required for this attempt is adapting these old buildings when the original function is no longer relevant or desired with new uses which is called as adaptive re-use.
The method required only a single empirical parameter, which related the 24 h minimum G0 (G0,MIN) to the 24 h maximum RN,S (RN,S,MAX) as G0,MIN = a × RN,S,MAX, and a = −0.31 was found by simple linear regression (p < 0.01).
Similar(30)
The method requires no smoothing.
The method requires only two parameters, alpha and T.
The method requires "tagging" components to be stored on servers.
The method requires caution, for some animal genera have changed their mode of life.
The method requires his actors to put an enormous amount of trust in Reygadas, particularly given the sexual content.
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