Antigen/epitope retrieval will restore this, but its success is very dependent on the method of retrieval and the method may have to be adjusted from antibody to antibody (or from target protein to target protein).
Due to the complex nature of treating patients with bladder cancer, the difficult course leading up to the surgery, and the method of retrieval of the survey, several items were missing at the time of data collection.
Since we want to do it in real time with a growing database, we need a fast method of retrieval.
Shen et al. (2015) used the method of image retrieval based on feature template matching to extract the collapsed building information.
These differences in cutoff levels, among different studies, may be explained by differences in the immunohistochemical procedures applied, such as the method of antigen retrieval, the immunolabelling protocol, and by differences in the scoring criteria of (clustered) Ki-67-positive cells.
This antibody has optimised specificity by using the method of microwave antigen retrieval on formalin-fixed, paraffin-embedded tissue.
In our study, cytoplasmic p21 WAF 1/ CIP 1 staining was evident as previously reported [ 33, 36, 37] and was based on the method of microwave antigen retrieval (Fig. 1b) using an alkaline buffer (Tris-buffered saline/EDTA, pH 9.0), rather than the acidic citrate buffer (pH 6.0) [ 17, 34, 35].
First, each of the nine antibodies was tested strictly in accordance with the manufacturer's recommended protocol, including the methods of antigen retrieval and the length of the primary antibody incubation time.
However, the methods of antigen retrieval from paraffin-embedded samples, the concentrations of antibodies, the time of incubation, as well as the amplification reagents vary and may significantly influence the final results [ 114, 116].
For structured materials such as shopping lists and colleagues' names one can use techniques such as the method of loci or spaced retrieval practice.
Hachemi and Alla (2008) presented an optimisation method of retrieval sequencing where the AS/RS was depicted by a coloured Petri net model.
