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The method of quantification followed closely the procedure outlined by Khan et al. (1997).
However, these two methods are quite different in regard to the method of quantification.
Because fluorescence is measured, the method of quantification is limited to fluorescent tracers and fluorescent colloidal suspensions.
The method of quantification is outlined in the Image J documentation: "Particle Analysis".
Essential to the process of understanding and reproducing these abnormalities is the method of quantification of flow scores.
The method of quantification used in the present paper is similar, but we have modelled the decremental number of cases over the years after the screen detection.
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The methods of quantification are outlined in the Image J documentation: "Image → Staks" and "Analyze →Measure".
It would also be helpful if more details about the methods of quantification were provided.
After reviewing Hayashi's four methods, we discuss two topics which have been pointed out as the problems to be solved in applying the methods of quantification.
Quantification was performed using the method of absolute quantification with the help of LightCycler Nano software version 1.1.
The method of relative quantification of EGFP mRNA via real-time PCR is as previously reported [ 33].
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