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The diagnostic tests have cut-off values that are set by the manufacturers for brain but in this study cut-off values were determined to take account of the method modifications and the different tissue type analysed.
To overcome this drawback of the method, modifications to the original FISH protocol have been put forward that significantly enhance the sensitivity of the assay [ 7, 8].
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The new chamber was compared with the traditional McMaster chamber in both cases using the McMaster method modifications [ 1- 3].
To verify the validity of this experimental method, modifications to the original experimental design have been made within the domain of this method.
Here we briefly discuss the relevant aspects of the method and modifications for gene expression interaction networks.
This work pertains to the method for modification of silicon (Si) wafer morphology by metal-assisted chemical etching (MacEtch) technique suitable for fabrication of antireflective Si surfaces.
The techniques include agar disk diffusion, broth dilution (macrodilution and microdilution), agar dilution and E test method (modification of the disk diffusion and the agar dilution method) [14].
The methods of modifications which are generally based on simple impregnation, sorption as chelates and chemical bonding have been discussed.
Among the reported methods, modification of gelatin with methacrylic anhydride (MA) stands out as a convenient and efficient strategy to introduce functional groups and form hydrogels via photopolymerization.
Materials and methods: Modifications of the peptide backbone, the chelator and the use of D- and non-naturel amino acids were proposed to improve the peptide tracer stability.
For clarity the method and relevant modifications are described in detail.
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