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The results showed that using the method investigated in this work, Au electrodes with a submicron-sized gap could be easily fabricated, with good uniformity and reproducibility.
We conclude that the method investigated in this work uses a straightforward protocol to enable efficient intracellular delivery of molecules with high cell viability using nanosecond-pulse laser-activated carbon nanoparticles for laboratory use and possible future in vivo applications.
The method investigated, hereafter called 'PRR-by-therapeutic area (PRR-TA)' restricts the background for comparison (b, d in Supplementary Table 1) to consist of drugs from the two respective therapeutic areas instead of all drugs in the EV.
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The methods investigated in this paper belong to the TE category.
The rank-based Wilcoxon, least squares (LS) procedure, and mle procedures complete the methods investigated.
The methods investigated are a 42% threshold, the Otsu method, and a deformable surface method based on Fourier descriptors.
In this study, the methods investigated were selected based on the known matrix interferences expected from honey.
All the methods investigated here are p-value based methods, the advantage of which is their flexibility and wide applicability.
Figure 2 shows the histograms of the minima of the cost function attained by each of the methods investigated.
The methods investigated here are likely to play an important role in identifying pathways that contribute to complex traits.
The methods investigated here, or simple modifications of these methods, could also be applied to other phenotypes, such as case-control status or time-to-event outcomes.
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