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Any sequences that contained possible CpG islands were removed; CpG islands were identified by following the method as outlined by Takai and Jones (2002).
To deal with censoring, we used the method as outlined by Baser and colleagues [ 12] wherein costs are recorded as a panel (monthly) dataset and inverse proportional weighting is used to derive an unbiased estimate of costs even if we have informative censoring.
Use the method as outlined above.
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This has been explicitly outlined in the Methods, as outlined above.
The relative expression among the different conditions (exercise or treatment groups) was determined using the ΔΔ C T method as outlined in the Applied Biosystems protocol for RT-PCR.
Central ECD was measured by noncontact specular microscopy (Nonconrobo, Konan Medical Inc., Hyogo, Japan), using the center method as outlined by the manufacturer's software.
Endothelial cell density was measured with a Konan Noncon Robo noncontact specular microscope (Konan Medical, Hyogo, Japan), using the centre method as outlined by the manufacturer's software or with a ConfoScan 4 confocal microscope (Nidek Technologies, Greensboro, North Carolina, USA) using the manufacturer's manual cell count software.
Common kits can cost as much as $4.28/sample, which is extremely expensive compared to $0.05/sample with the PI method as outlined.
Paraffin sections of the small intestinal mucosa, 3 µm thick, were used for immunostaining with Ki-67, performed by the same method as outlined above.
Total ribonucleic acid (RNA) was extracted from approx. 100 mg ground plant tissue using the phenol/chloroform method as outlined in Burton et al. [ 31].
To solve this low SNR problem, an obscuration in the lens system of the device in Fig. 2(a) is introduced that accomplishes a similar function as the conventional method, as outlined in Fig. 1(b).
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