Sentence examples for the medium was substituted from inspiring English sources

The next day, the medium was substituted with DMEM/F12 containing 0.1% FBS with or without siRNA to render the cells quiescent.

The medium was substituted with Knockout-DMEM (Kon on d2 that contained all the additives and Knockout serum replacement (Invitrogen) in place of FBS and was supplemented with (KR) or without 100 nM all-trans retinoic acid (RA).

The medium was substituted by fresh DMEM, 10% FBS (3 ml).

After 1 h incubation, the medium was substituted with ordinary growth medium.

For these measurements, cultures were washed and the medium was substituted with HEPES-buffered saline medium before stimulation.

The following day the medium was substituted and then collected after 24 h, clarified from cellular debris by centrifugation and added to the cells of interest.

When indicated, the 2% glucose of the defined medium was substituted by glycerol (in fact, 2% glycerol and 0.2% glucose).

After 15 min, the incubation medium was substituted for the same solution containing collagenase only.

The next day, the cells were serum-starved for 16 h before the FCS-free medium was substituted by complete culture medium supplemented with kinase inhibitors as indicated.

To differentiate L6 myoblasts into myotubes, the culture medium was substituted with DMEM plus 2% horse serum after the L6 myoblast cells became confluent.

After 12 hours incubation, the hypoxia medium was substituted by normal medium with or without DG and the cells were incubated for another 4 hours before performing various assays.