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Furthermore the medium was modified to induce neural maturation.
The pH of the medium was modified using oxalic acid and sodium hydroxide.
Further, the composition of the medium was modified.
After reaching confluence, the medium was modified to include ascorbic acid at 50-μg/ml [ 51].
Viscosity of the medium was modified by adding methylcellulose (M0512; Sigma-Aldrich; St . Louis MO; approximate molecular weight 88,000) at concentrations 0%, 0.2%, 0.4%, 0.5% wt/vol.
The medium was modified by replacement of the natural abundance amino acid l-alanine by a combination of l-[3-C]alanine and d-[1-C]alanine (each, 0.1 g/L), the natural abundance glycine by [2-C]glycine, and the natural-abundance lysine by l-[5-F]lysine.
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In this paper, taking into account the experimental characterization reported in [10], we proposed that in some materials the nonlinear response can change for each position: something in the medium is modify that affects to the beam in a different way than in the previous position.
Finally, the P-YEM medium was modified by the addition of 1 mg.l-1 of vancomycin to obtain the final medium, named PV-YEM.
Based on this finding, the culture medium was modified to increase intracellular ATP concentration by cell growth inhibition.
The GS2 medium was modified for α-cellulose co-cultures to contain 10 mg/L Ergosterol and 420 mg/L Tween 80 and 4.09 g/L Glutathione in place of 2.33 g/L Cysteine HCl H2O (termed ETGtGS2).
For the study, standard TAP medium was modified by eliminating acetic acid and supplementing with 1 g NaNO3 L-1 and 4 μg vitamin B12 L-1, and this formulation was termed co-culture medium.
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