Exact(6)
Based on the medium obtained from shake flask optimization experiments, a scale-up fermentation was conducted in a 5 L bench fermentor.
The medium conditioned by the aneurysmal segment of the abdominal aorta contained more cf-DNA than the medium obtained by incubation of the adjacent aortic segment from the same rat (thoracic aorta, Figure 11A).
GAGs were precipitated from the medium obtained from days 4 7 with Alcian Blue [ 29].
For the total release of proteoglycans, the GAG in the medium obtained from days 4 7 were precipitated and stained with Alcian Blue as described above.
In this case, a Green's function of the medium obtained with the high order perturbation theory could be used to improve the forward solver [ 26– 28].
After 72 h of incubation, the medium obtained from FHUVECs (n = 10) and MHUVECs (n = 10) (30,000 cells/cm) was collected to measure H2O2 using a commercial kit (Colorimetric Hydrogen Peroxide kit, Stressgen, Milano, Italy) following the manufacturer's instructions.
Similar(54)
The band diagram structure and damping ratio are computed for different repetitive structures, based on the micropolar viscoelastic response of the effective medium obtained by the homogenization of the initially discrete lattice architecture.
To investigate the effects of enzastaurin treatment on the production of the main angiogenic growth factor VEGF, the cell lysate and the conditioned medium obtained from the various cell lines treated with enzastaurin were collected and analysed for the presence of VEGF by ELISA assay.
The reaction medium obtained from the reduction process of indigo as previously described was used as a dyebath.
The levels of the other cytokines secreted in CM by C-26 cells, such as INF- γ, IL-10, TNF- α, and IL-12, were not significantly different compared with the control medium obtained from the C2C12 myogenic cell line.
The reaction medium obtained resulted from the reduction process was used as a dyebath at 60°C.
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