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After 72 h in normoxia, the pH of the medium dropped from pH 7.8 to 6.7 for NT2 and from pH 7.6 to 6.4 for NCCIT.
In hypoxia, the pH of the medium dropped only from pH 7.7 to 7.6 for NT2 and from pH 7.5 to 7.3 for NCCIT in the same time span.
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It was also observed in this work that the pH of the fermentation medium dropped to a value below 5.6 from the initial value of 7.0 ± 0.2 after 48 h.
GFP –CgCTA1 became also induced after the glucose concentration in the growth medium dropped below 0.03% (Fig. S2A).
For example, in our experiments, the nitrate level on the 1 mM medium dropped below 0.5 mM after 1 week and was near 0.1 0.2 mM after 3 weeks (data not shown), with relatively healthy and reasonable-sized plants.
The number of detached cells in the rinsing solution and medium dropped steeply from 96.0 ± 1.9 × 10 to 49.5 ± 2.4 × 10 within 2 hours.
Because glucose concentration in the experimental medium drops below 25 μg/ml as the population grows, competitive ability at lower concentrations was likely favored, even if only briefly [ 21].
If the intravascular concentration of contrast medium drops under the interstitial concentration level, diffusion takes place in opposite direction until all contrast is eliminated: this is called 'wash-out' [19, 20, 21].
The medium was dropped onto a glass slide.
Once placed, the medium was dropped onto the center of the sheet to spread folded parts of the transferred engineered cardiomyocyte sheets.
The medium pH dropped and cytoplasmic acidity increased significantly in both MCF-7 cells and MDA-MB-231 cells when CtBP was knocked down.
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