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After 6 h, the media was changed.
After 4 days the media was changed to neurobasal without horse serum.
The top half of the media was changed every day for two days.
Half of the media was changed every 2 3 days to allow culture conditioning.
Cells were incubated with the nanoparticles and the media was changed just prior the irradiation.
The media was changed thrice a week until they reached confluence.
The media was changed to fresh RPMI 1640 media with 10% PBS and incubated for 12 h.
The media was changed daily.
The media was changed prior to the infections.
The media was changed on the third day.
The media was changed 24 h post-transfection.
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