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Endosomal enrichment was defined as the ratio of the mean intensity in the ROI divided by the mean intensity in the whole cell.
Here we measured the standard deviation of pixel intensity and the mean intensity within each cell.
Each channel was converted into the eight-bit grayscale and the mean intensity was calculated.
The mean intensity of all cells at each timepoint is shown, and error bars represent SD.
The mean intensity of the green channels in the nuclear region (DAPI positive) was quantified by LAS X (Leica).
The mean intensity of EGFP was quantified by drawing a free-hand region of interest (ROI) around the cell soma using ZEN 2012 SP1 software.
3D voxels centered in mask coordinates are selected by equal-width binning-based discretization of the mean intensity.
The mean intensity is expressed in counts per second (cps).
In order to satisfy Property 1, the relationship of the mean intensity values can be illustrated in Figure 2. Figure 2 Relationship of the mean intensity values.
The mean intensity of each cell was determined by drawing regions of interest around each cell.
Green circle is the mean intensity by the Tsunakawa Shaw method.
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