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The mean depth for the filtered SNPs was 3.3.
The mean depth for each accession was about 0.50 × on average (ranging from 0.27 × to 0.96 ×) (see Additional file 8).
The filter has also removed the relationship between the estimate of self-relatedness and the mean depth for the animal (Fig. 8).
There is some dispersion of the mean depth for the whole PANDIT dataset observed in Figure 2, which is attributable to imbalanced bifurcations in some specific trees.
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To demonstrate the expected higher coverage of MiSeq with WGBS, we calculated the mean depth of coverage for each CpG locus, within each amplicon, for each group (Fig. 5).
To describe potential GC bias in sequencing runs, we calculate the mean depth of coverage for each GC content bin (0 100 representing 0 100% GC composition) for a series of windows along the genome (or, in the case of targeted sequencing experiments, within targeted regions).
Before their removal, the mean depth of coverage for targeted regions was 383 reads, but after duplicate removal the mean depth amounted to 110 reads with base phred quality scores of at least 17.
The mean depth of coverage for sequence reads to the EAV-HP LTR was 12.58 ± 1.08 in Ethiopian chickens, 7.38 ± 2.33 in Pirbright lines, and 16 ± 2.83 in the SRA data, respectively.
Overlapping features were merged using the mergeBed tool from BEDTools and filtered to exclude those supported by a read count (RC) less than 25%% the mean depth of coverage for the sample (further details in Additional file 2: Supplementary Methods).
Student's t-test showed a statistically significant difference (P < 0.05) between the mean depth of excision for the two study groups; we therefore demonstrated that the risk of preterm delivery is higher when cone depth exceeded a cutoff value of 1.5 cm (O.R. 7.143, 95% CI 1.37 37.228).
The peak frequency of water surface elevation occurred at 4.6 h, which corresponds to the first mode of 112 km seiche oscillation for the mean depth of Leyte Gulf.
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