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Within this contribution we investigate the influence of this cell-to-cell variability on important process variables like the maximum virus yield.
Results are recorded as the reciprocal of the maximum virus dilution that produced agglutination.
The current results from groups D & E (Table 1) indicate that 1 µg of F-particle may induce the maximum virus neutralizing antibody responses in the mouse model.
In all cases, escalation of the virus dose was possible without that a dose-limiting toxicity was observed and the maximum virus dose that could be administered was based on manufacturing capabilities (Ganly et al, 2000; Mulvihill et al, 2001).
As it has been reported in MDCK cells that lactate concentration of 8 mM (or 0.7 g/l) at the time point for infection can reduce haemagglutination unit by a factor of two [ 66] and the addition of ammonium chloride to 20 mM was reported to block infection [ 67- 69], the high lactate level we observed may have some influences to limit the maximum virus titres achieved with these medium.
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It is shown that a medium exchange is not required for infection and that maximum virus titers are obtained for 1 × 10-6 trypsin units per cell. 1 L bioreactor cultivations showed that 4 × 10 cells/mL can be infected without a cell density effect achieving titers of 1 × 10 virions/mL after 24 h.
Briefly, infected Vero cells were harvested at the time of maximum virus induced cytopathic effect.
Additionally participants were recruited at the time of maximum virus circulation.
In the context of an in vivo application, it would be extremely important to initially protect immunoliposomes from macrophages, in order to provide enough time to redistribute through the body and achieve maximum virus binding.
One primary animal infected with the NJ95COE yielded a maximum virus titer of 103.05 TCID50/ml on PID 4, and positive swab samples were collected on PID 4 and 7. Virus was not collected from swab samples of the second primary animal.
The mice infected with rZH501-M847-G alshowedwed rapid virus replication in the liver, and yet the maximum liver virus titer was approximately 10 times lower than that in the mice infected with rZH501-M847-A, ZH501 and the mixture of rZH501-M847-G and rZH501-M847-A.
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