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In the culture without pH control condition, the maximum viable cell concentration (1.5 × 1012 cfu/mL) was 8.3 times higher than the culture with pH control condition (1.8 × 1011 cfu/mL).
The maximum viable cell concentration of P. acidilactici reduced when NaOH was added because the additional ions from NaOH increased the osmotic pressure of the medium, causing reduced cell growth (Cui et al. 2016).
The maximum viable cell concentration obtained in constant fed-batch fermentation at a feeding rate of 0.015 L/h was 6.1 times higher with 1.6 times reduction in lactic acid accumulation compared to batch fermentation.
After having reached the maximum viable cell concentration of 1.3 × 10 cells/mL, cell death started.
The 16-mg/kg dose was considered to be the maximum viable dose.
Although an MTD was not reached, the dose of AS1402 was not escalated above 16 mg/kg, which was considered to be the maximum viable.
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Of all the three agitation speeds studied, 300 rpm showed the highest maximum viable cell concentration (1.8 × 1012 cfu/mL) with improvement of 1.5 times and 1.8 times compared to 200 (1.2 × 1012 cfu/mL) and 400 rpm (1.0 × 1012 cfu/mL), respectively.
As summarized in Table 4, the highest maximum viable cell concentration was always achieved in SFM* #4.
Similar to the results of maximum viable cell concentration, yield and productivity, the same trend was obtained for lactic acid production, yield and productivity, where the results were slightly higher in facultative condition (12.98 g/L, 0.91 g/gGlucose and 0.53 g/L h respectively) compared to anaerobic condition (11.32 g/L, 0.86 g/gGlucose and 0.46 g/L h respectively).
Two siRNA-introduced cell lines grew logarithmically, with a slight difference in the specific production rate, maximum viable cell density, and the day upon which the viable cell density reached the maximum level; two siRNA-introduced hygromycin-resistant descendants showed slightly slower growth and death rates than those of the parent cells.
However, the time taken for the culture to reach maximum viable cell concentration was 6 h longer in the fed-batch fermentation with resin compared to without resin.
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