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The effectiveness of siRNA was characterized by measuring the IC50 values in cell culture and the maximal extent of target suppression.
It was shown that the maximal extent of the MAZ caused by an underground structure is reached approximately 20 years after construction.
In addition, protein-loaded microspheres were subjected to the laser sintering process and the bed temperature of the part was chosen to be 35 °C without further preheating to protect the bioactivity of BSA to the maximal extent.
At this time the activity had remained constant for several hours (see below); thus, the results indicate the maximal extent of reactivation.
The maximal extent of cleavage (which is ∼40%) requires only 200 µM GlcN6P and an incubation time of ∼1 min (Figure S3b).
To classify the maximal extent of level changes in individual CD patients over time we identified the samples with the maximal change in QSS (increase or decrease) compared to the first available sample per individual CD patient during follow-up.
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Although bacterially expressed AMPK complexes can be activated >100-fold in cell-free assays by stoichiometric phosphorylation of Thr172 (Suter et al., 2006), it remained unclear whether the degree of Thr172 phosphorylation is ever close to this maximal extent in intact cells.
The results were the same for the overall depth of all lesion zones as well as for the minimal and maximal extent.
Further, slow kinetics of the organic film spreading at the interface to its maximal extent is considered as the rate determining step, which could be a consequence of the attenuated effect of potential at the modified interface, stronger intermolecular interactions and reorganization of molecules in the film.
3. Repeat the preceding step until the tree reaches its maximal extent (no pruning).
The criteria are formulated, which allow determination of the sets of electrophysical and design parameters of the diode temperature sensors (DTSs) providing either maximal extent of the temperature response curve (TRC) or the maximal sensitivity of DTSs.
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