The mating between AH109 transformed with a plasmid expressing the BD-Lamin fusion protein and Y187 transformed with a plasmid expressing AD-T was used as the negative control.
The mating between AH109 transformed with a plasmid expressing the GAL4 binding domain (BD -p53 fusion protein and Y187 transformed with a plasmid expressing the GAL4 activation domain (AD)-T (SV40 T antigen) fusion protein was used as the positive control.
One may argue that the Scandinavian wolf population is extreme in its level of inbreeding, as it was founded by only three individuals [12], [16] and now having an average inbreeding coefficient of 0.25, corresponding to the mating between full sibs.
For instance, the mating between D. simulans males and D. melanogaster females results in a dramatic larval death of the male offspring that is accompanied by a reduction of the brain and lack of imaginal discs [7], [8], while the surviving adult females are sterile [3], [9].
Since the present model did not allow multiple infections of a single host unit, the mating between strains on the same host-tissue was simulated as the mating between strains on the same cultivar.
Because this model does not allow multiple infections of a single host unit, the mating between strains on the same host-tissue was simulated as the mating between strains on the same cultivar.
