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The material was fixed at 2.5% glutaldehyde and 2% paraformaldehyde solution; post-fixed at 2% osmium tetroxide; dehydrated with increasing ethanol concentrations, and included in epoxy resin.
For this, the material was fixed in glutaraldehyde 2.5%, soon after it was submerged in buffer phosphate during 24 hours, then fixed in osmium tetroxide (OsO4) 1.5% at 2°C for 3 hours, dehydrated in an upward series of acetone and embedded in Spurr's resin.
The material was fixed in a 10% formalin solution and embedded in paraffin.
In the control sections, where the material was fixed without the addition of pyroantimonate, electron-dense precipitates did not occur.
All the material was fixed and stored in 4 % solution of paraformaldehyde in phosphate-buffered saline (PBS).
To enable an ultrastructural analysis, the material was fixed in 4% buffered paraformaldehyde, postfixed in 1% OsO4, dehydrated with alcohol in increasing concentrations, and embedded in Epon-Araldite.
Similar(50)
The amount of time in which to learn the material is fixed, but the amount of learning varies significantly.
The materials were fixed in FAA and stored at 4 °C for 24 h.
The diagnostic material was fixed in the routinely-used fixative.
In this study, the culture material was fixed with trichloroacetic acid and then stained for 30 min with 0.4% (w/v) sulforhodamine B mixed with 1% acetic acid.
The remaining material was fixed for immunohistochemical analysis.
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