This strongly contrasts the 252 miR-769-3p/-450b-3p seed matohes unique thethediteded 3′-UTR data set (Table 1 and Fig. 2A).
In the molecular function, these matched unique sequences were clustered into nineteen classifications.
With the Gene Ontology (GO) classification, the 593 matched unique sequences were classified to three functional categories: molecular function (Fig. 5), biological process (Fig. 6) and cellular component (Fig. 7).
The 880 matched unique transcripts were selected as a subset of the 5,760 EST fragments and subjected to further BLAST analysis to obtain the three highest scoring alignments.
The 593 matched unique sequences were clustered into fourteen categories (Fig. 4), using Cluster of Orthologous Groups of proteins (COG).
Of all the 593 matched unique sequences, 153 unique sequences (28 contigs and 125 singlets) were homologous to genes related to immunity in other organisms, and this was the first report of these immune genes in E. carinicauda.
: The percentage is calculated by dividing the number of unique sequences (9622) by the number of matched unique sequences (2027 and 2026, respectively).
Thus, this failure may be due to the repetitive nature of the vls locus and/or its frequent cassette switchings [ 20, 21], which makes the generation of matching unique primers impossible.
RNA-Seq data showed that 82.5% of the reads that matched unique regions could be mapped to known coding/UTR regions, 15.9% to intergenic/unknown UTR sequences, and 1.6% mapped to genomic regions encoding rRNA and tRNA.
There were large variations about the number of matching unique miRNAs in these three different stages of taproot thickening, 18,078 (stage1), 36,239 (stand23,604 23,604 (stage3) unique miRNAs reads were matched to known miRNAs, respectively, implying that miRNA-mediated gene silencing is involved in the regulation of radish taproot thickening.
