In the EGF repeat domain at the extracellular side, mutations were found across the majority of the EGF repeats, but none of the mutations led to a gain or loss of a cysteine residue, which is the defining mutation for NOTCH3 in CADASIL [ 33].
After incubation at 37°C for 3 h, the majority of Texas Red-EGF was essentially degraded in A431 cells as well as in A431 cells expressing HA Tom1L1 and Tom1L1 SH3 with trace amounts on the plasma membrane and in intracellular punctates.
Collectively, the wide expression of Isl1, SM-MHC, nestin and Smtn-B in the majority of the early EGF-induced ATMCs is an unequivocal evidence of their gain of traits of VSMCs progenitors, a finding which is in strong support that adult ATMCs retain vasculogenic potential similar to embryonic MCs.
The majority of genes in the EGF gene set are functioning in the second messenger system while the nuclear localization pathway only involves EGFR.
The majority of inhibitors tested on EGF-stimulated PMC42-ET cells were efficacious at reducing cell count; however, nearly every inhibitor tested had an IC50 for reducing the number of vimentin+ cells well above pharmacologically relevant concentrations (Table 3 a- e), thus off-target effects are likely.
We have investigated three human lung squamous carcinoma cell lines (NX002, CX140 and CX143) and have shown, using an antibody (EGFR1) directed against the EGF receptor, that the majority of cells in all three lines express the EGF receptor.
This indicates that vesicular recycling between the PM and the RE maintains a steady state with the majority of EGFR at the PM, whereas EGF stimulation redirects self-associated EGFR towards lysosomes thereby depleting it from the PM.
In mice lacking EGF-receptors, the majority of insulin-positive cells remain associated with pancreatic ducts in the newborn period.
In fact, the majority of lymph node positive patients display elevated HB-EGF plasma levels.
Polarized extensions of lamellipodia toward higher EGF concentrations were observed in the majority of cells [see Additional File 5].
