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To sum up, when the spindle rotation speed is 390 r/min, the main spindle errors basically do not influence the frequency domain error specifications.
To perform the accuracy test, a three-degree-of-freedom (3D) measuring probe is installed in the main spindle and a base plate is fixed on the turntable.
Under the process parameters with the spindle rotation speed of 390 r/min and the feed rate of 0.06 mm/s, however, one can make the spatial frequencies induced by the main spindle errors and NVIE avoid the evaluation range of middle-frequency band (i.e., enter into the evaluation range of low-frequency band or high-frequency band), eliminating the need to meet the PSD requirement.
(B ) Quantification of the area of the main spindle region.
(D ) Spatial distribution of α-tubulin in the main spindle region, as revealed by the mean standard deviation (SD) between the per-pixel fluorescence intensity values in the main spindle region, normalized to the mean fluorescence intensity of the main spindle region.
The main spindle region was defined on the basis of the cell body shape and the centrosomal region (both revealed by α-tubulin immunofluorescence) and the apical- and basal-most (peri- centromeric foci (DAperi- centromeric focire 2 A ).
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Likewise, our results for nod confirm that the nonexchange chromosomes that are ejected from the main meiotic spindle are mostly unable to reassociate with it by metaphase arrest, and that those chromosomes that fail to do so are eventually lost.
However, the tumor cells were mainly intravenous inside and outside of the stomach wall and the main component was spindle cells.
Since defects in planar spindle alignment have been shown to cause apoptotic cell death and delamination in wing disc epithelia [ 25 27], and SakOE neuroblasts also showed spindle mispositioning [ 6], we analyzed spindle positioning by measuring the angle formed between the main axis of the spindle and the apical surface.
We reasoned that if this balance of cytoskeletal forces was the main driver of apicobasal spindle position, then removing both F-actin astraltral microtubules should randomize spindle position.
We believe that this asymmetrical SPB defect is the main reason for monopolar spindle phenotypes observed in this mutant allele.
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