The main specificity of the proposed methodology lies in the algorithm for calculation of stress histories in the stirrups and in the web concrete.
The main specificity of the insect NMJ, compared to the vertebrate NMJ is the presence of glutamate as a neurotransmitter instead of acetylcholine.
The main specificity of BaliBase was to address a wide range of different issues related to multiple sequence alignments.
In order to be able to compare HLA genotypes/haplotypes between different data sources or typing methods, all HLA types were converted to pooled alleles (see supplementary material), if necessary, to the serological main specificities according to the official HLA nomenclature [17].
The two main specificity issues that are seen in research are the use of able bodied subject groups and using laboratory ergometers.
The reason to predict both sequence-specific and non-specific binding residues is that the main determinants of specificity are the unfavorable contributions of "wrong" base pairs and specific binding will also require a large non-specific contribution to the binding free energy to achieve sufficient binding affinity [ 38].
Because the seed region is the main determinant of specificity, it is believed that the members of a given miRNA family regulate a very similar set of target mRNAs [24].
Overall, while all three clusters simultaneously encode specificity on different parts of the substrate peptide, by shaping the active site in a cumulative and non-linear fashion, cluster 1 appears to be the main driver of specificity.
Even so, a peptide comprised of all P4 P2′ residues requires millimolar concentrations to affect proteolysis of TatA by AarA, indicating that even in this context recognition sequence binding is not the main determinant for specificity.
Even at millimolar concentration (∼1000× the substrate concentration), the peptide did not block rhomboid proteolysis of TatA or APP + Spi7 substrates, independently suggesting that sequence-specific binding is not the main feature of rhomboid specificity.
