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The long variant of the gene seems to confer emotional resilience.
Full-length cloning demonstrated expression of the long variant in endothelial cells.
Since the long variant of mSIRT3 consists of 334 amino acids, we calculate that the longer variant should be 37 kDa.
In order to test for this, we used (1−p l, where p is the frequency of the long variant and l is the length of the indel as explanatory variable (see Text S1).
For instance, if the chimp sequence is the long variant this implies that either a human deletion or a chimp insertion occurred and we need the macaque sequence to resolve this (see Figure 1).
The data suggest that the long variant, CRACR2A-L, is the variant of endothelial cells.
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That indicates a slight over-representation of the long variants in happier people.
All Mca1 variants exhibit the Q/N-rich regions, while only the long variants Mca1451/453 share an extra stretch of 19 amino acids at their N-terminal end.
Further examination of the samples from that study, show that all short DCLK1 variants are induced by BDNF but not the long variants (see figure 1B).
Our antibody was raised against the complete CaRF protein and will immunoprecipitate both forms of CaRF, and importantly both the short and the long variants of CaRF are absent in our KO mice [15].
The short isoforms lack the C-terminal protein binding sites (CtBP) which convert the long variants of TCF7L2 into a repressor [ 37].
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