The libraries from susceptible infected trees showed very limited transcript diversity and were chiefly composed of PR proteins, such as basic PR-1, PR-2 (beta-1,3-glucanase), PR-3 (class I, IV chitinase), PR-4 (chitinase type I & II), PR-5 (thaumatin-like protein), PR-6 (type II proteinase inhibitor family protein), and antimicrobial peptide.
Unfortunately, there is only limited transcript data available from conidia at the stage of penetration, but maybe the larger CSEPs (300 400 amino acids), belonging to the three largest families and in general showing low transcript levels have functions at the earlier stages during infection.
Because of limited transcript information on Acanthaceae family species, only ~4%% of the transcripts showed similarity with the annotated sequences of the Acanthaceae family species.
This observation is subject to considerable uncertainty due to limited transcript coverage, but human and murine periostin are compelling examples, having substantial amounts of transcript evidence.
Smaller samples were taken 1-3 times per day and used for limited transcript analysis with TRAC, dry weight and enzyme activity measurements.
It also suggests that limited transcript processing may have contributed to mstn2b nonfunctionalization. Previous studies revealed divergence within gene promoters while the current studies provide evidence for relaxed or positive selection in some coding sequence lineages.
The reads matching conserved genes and UniGene/UniProt sequences were reassembled and mapped with T. aestivum transcriptome sequences, giving 982 high-confidence gene models on 5DS and 2,165 on 5DL, along with a smaller number of low-confidence gene models with more limited transcript coverage (332 and 810 respectively).
The higher percentage of normal-specific AS events in mouse is explained by the limited cancer transcripts available for this species (Table 1).
One commonly used approach for setting up such "ground truth" is by spiking in bacterial synthetic transcripts with known concentrations in series of dilutions over a large dynamic range [ 22], however, the limitation of this approach is that the information is asserted from very limited transcripts, and it is also very prone to experimental artifacts.
The limited magnitude of transcript changes by miR-499 suggests that the hypertrophic phenotype observed was not due to gross gene expression disruption.
The limited correlation of transcript and protein expression is particularly notable in the study of human clinical samples.
