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We compute the likelihood of assignment of a read to all potential samples of origin, assign each read to the most likely sample and compute the uncertainty of the assignment.
The likelihood of assignment, ln P(D), increased from K = 1 to 7. It then fluctuated, and the highest value was obtained at K = 9.
The likelihood of assignment of a validated status was based on the site-specific validation rate of the presumptive events that were reviewed.
Similar(56)
However, increasing database size also makes it potentially more difficult to assign taxonomy at genus and species levels as the likelihood of ambiguous assignment increases due to larger number of competing sequences in the search space.
The contribution of each variable to the likelihood of cluster assignment was determined.
This cluster value does not represent the level of relatedness based on a genetic cross between two individuals but rather it is the likelihood of population assignment.
The analysis was used to discriminate who out of the potential victims had actually been infected by the common source, to provide an individual assessment of the likelihood of this assignment, and to obtain an estimate of the date of infection for each patient.
This restriction was made in order to reduce the likelihood of incorrect assignments by the BLASTN procedure (see Additional file 4: Table S3, cluster BLASTN assignation sheet).
The likelihood of the assignments was evaluated for K varying from 1 to 9, and the reliability of the K clusters was tested for these values and variances among the trials; ΔK was also used to assess the true K number [ 46].
The likelihood of the multilocus individual genotypes occurring in each population was estimated based on their observed allele frequencies and an individual was assigned to a reference population for which it had the highest likelihood of assignment.
To reduce the likelihood of false-positive assignments due to low-level similarity, we performed an empiric analysis on sequence-comparison data to define incrementally more stringent thresholds.
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CEO of Professional Science Editing for Scientists @ prosciediting.com