Sentence examples for the libraries were normalized using from inspiring English sources
Read counts of the libraries were normalized using the Upper-Quartile Normalization method [ 19], and were then loge (ln) transformed, yielding transformed normalized counts.
The libraries were normalized using the SAGE2000 software.
The libraries were normalized using double-stranded nuclease to digest high copy double‐stranded DNA during re‐association after denaturation and then prepared for sequencing as described by Illumina [ 31].
The SuperSmart cDNA Synthesis Kit (Clontech, CA) was used to synthesize cDNA, and the library was normalized using the Trimmer kit (Evrogen, Moscow) that reduces the quantity of the most abundant cDNA copies.
Reads of the eight libraries were normalized using the upper quantile normalization approach.
Libraries were normalized using the TRIMMER DIRECT cDNA Normalization Kit (Evrogen) and were carried out essentially as described in the user manual.
Libraries were normalized using the trimmed mean of M values (TMM) normalization method in the NOISeq R package (Tarazona et al. 2011) before assessing dosage compensation.
In summary, 4 out of the 15 full-length cDNA libraries were normalized using this approach.
cDNA libraries were constructed using SMART method (Clontech, Mountain View, CA) and the resulting amplified, double-stranded libraries were normalized using a double-strand nuclease (Trimmer, Evrogen, Moscow, Russia).
cDNA libraries were normalized using Trimmer cDNA normalization Kit (Evrogen).
Our first library was used for EST discovery; therefore, this library was normalized using the Trimmer-2 cDNA normalization kit (Evrogen) to minimize over-presentation of some abundant transcripts.
