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The level of producing nitric oxide (NO) by ASC and ELC was measured by colorimetric (Nitric Oxide Assay Kit, Abcam) according to the user protocols.
The overall conclusion that can be drawn from biochemical analysis of transgenic seeds is that after three seasons (2006-2008) of plant cultivation in field the level of producing compounds as the result of three genes overexpression is stable.
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Dynamic single-cell culture arrays of isolated cells have enabled to determine the level of produced or secreted proteins but do not simulate the physiological conditions of a 2D cell culture [21, 27].
The extent of DNA damage depends on the level of produced ROS and the robustness of innate antioxidant defenses.
In this study, we found that Cav-1 deletion induced an increase in the level of produced ROS, and this increased production of ROS could be the reason for the loss of HSC function.
For the same reason, as additional expression of ispF increased the level of produced carotenoids significantly, it is suggested that ispF has a larger contribution to the flux control in the MEP pathway than ispD.
In vitro and in vivo assays showed that HSCs from Cav-1−/− mice decreased the ability to self-renew and reconstitute hematopoiesis, and Cav-1 deletion disrupted the quiescence of LSKs and increased the level of produced ROS.
One possible explanation for this is that the level of produced His-TAT-Apoptin or His-TAT-Apoptinopt did not reach the threshold value where there was a protein burden and therefore no growth arrest occurred.
The levels of produced carotenoids, diaponeurosporene and diapolycopene, were used as indication of the role of the various enzymes on the flux of the MEP pathway.
Oxidative stress defines disequilibrium between the levels of produced ROS and the ability of a biological system to detoxify the reactive intermediates [ 70].
The sleeping sensation reduces the level of vigilante producing danger situations and increases the probability of an accident occurring.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com