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The leading processes of these neurons tend not to branch and remain club-like as they migrate (Fig. 5).
We immunolabelled embryonic cortex (E16) with interneuron specific anti-calretinin or calbindin antibodies and analyzed the orientation of the leading processes of these neurons.
The pathfinding of the leading processes was recorded in collagen matrix when axons were located out of the rhombic lip explant.
Interestingly, in wt explants, the maximum outgrowth was reached with an intermediate concentration of collagen (50%), whereas when the collagen was diluted (25%) or concentrated (75%), the total outgrowth of the leading processes decreased to similar levels (Fig. 6C).
When cultured with soluble Netrin-1, the quantification of the total outgrowth of the leading processes by TUJ1 immuno-staining and thresholding revealed that their growth was reduced about 3 times in TTL−/− explants, after 3 days in vitro (3 DIV) (Fig. 3A, C, D, F and G).
Although radially oriented interneurons in the cortical plate make multiple contacts with radial glia, the orientation of the leading processes of these cells are always not aligned with radial glia processes in fixed tissue preparations, leading to the suggestion that these GE derived interneurons probably do not migrate along radial glia [16].
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Although the cell body was immobile, the leading process continued to grow (Tsai et al., 2005).
Myosin II activation happens periodically at the rear of the nucleus where it promotes actomyosin contractions to push the nucleus toward the leading process (Godin et al., 2012).
In vivo suppression of JNK disturbs the leading process morphology of migrating neurons and the pial surface-directed neuronal migration (Kawauchi et al. 2003).
We observed a partial rescue after taxol treatment assessed by the length and the number of leading processes in the double-mutant cultures (Fig. 8).
For each genotype the number of leading processes was counted and the data represent mean ± SEM.
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