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The largest viable metabolism contains all n = 51 reactions.
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The present experiments were designed to determine the combination of hypothermic and alkaline conditions that yield the largest number of viable neurons dissociated from intact DRG maintained ischemic for up to 4 h.
Glucose has the largest set of viable genotypes, followed by succinate and acetate.
Inspection of Figure 5(a) reveals that, initially, the in-house AAO membrane had the largest number of viable cells attached to its surface.
As these cells formed multi-focus in the wells, we performed the clonal isolation by limiting dilution in a 96-multiwell plate picking up cells from the well that contained the largest number of viable cells.
Figure 11-a already hints at this observation, because it shows that the largest density of viable parameter points occurs in regions of parameter space where both k11 and k12 are high.
Annexin V/PI staining in the control group showed a large viable cell population (marked as PI - AV-) and a small amount of early apoptotic (PI - AV+), late apoptotic (PI + AV+), and dead cells (PI + AV-).
However, culturing large, viable three-dimensional constructs is an engineering challenge, since the viability of cells within the construct is heavily dependent on adequate oxygen and nutrient delivery.
Using these targets, gap analyses should be conducted to identify ecosystem types currently under-represented in the protected area network (or within degraded protected areas that have lost their conservation value) and new areas required for priority species that have insufficient habitat to maintain large viable populations.
Hence, robustness is associated with larger viable volumes.
As expected, large viable cells were negative for both, C1q and annexin A5.
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