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At long period, H 800 and f 0 are providing the largest differentiation.
The largest differentiation expressed by FST values was observed between the Turopolje TL and Turopolje AUT populations (FST = 0.26).
The degree of genetic differentiation was smallest between the Danba and Xiaojin populations, whereas the Heishui and Jiulong populations had the largest differentiation.
The largest differentiation between pairs of subpopulations was found between the Western-Central Europe and the Eastern groups, for both Ks = 3 and 5, with D est = 0.217 and 0.256 respectively).
These general observations held for all V A where capture was observed, with capture occurring at smaller gates with lower V A. The largest differentiation between strains was observed at V A = 1000 V.
Considering only breeding populations, eastern Spain (SpE) showed the highest number of significant pairwise comparisons (seven out of 12), while the largest differentiation among populations was found between western Spain (SpW) and central France (PLO) (SpW-PLO: ΦST = 0.216).
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However, group 3 displayed the largest differentiations (0.28 < F ST < 0.45; P < 0.001) in agreement with the early distinction of this cluster in the first (K = 2) analysis of STRUCTURE.
F ST analyses among the six genetic groups rendered an average genetic differentiation of 0.22, but again group 3 displayed the largest differentiations from the rest of clusters (0.24 < F ST < 0.42; P < 0.001) and the lowest genetic diversity (Table 3).
The large differentiation of the targets means that deviations from the model can be observed; however, the trend is obvious.
These two clusters are separated by an average corrected mtDNA sequence divergence of 3.02 % (±0.36) and this large differentiation is further supported by the Neighbor-Net tree (Fig. 3c).
Analysis of molecular variance (AMOVA) showed that A genome had the largest genetic differentiation and D genome the lowest.
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