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Does the large rate enhancement characteristic of biological catalysis result in different transition states relative to solution reactions?
By combining transient response experiments with forced concentration cycling, we were able to determine the reactive surface intermediates responsible for the large rate enhancements observed during periodic operation.
This is especially relevant for upcoming stories where the large rate of new submissions means a given story remains near the top of the recency list for only a few minutes.
Unlike PS1 and PS2, in PS3 the large rate of For3p retrograde flow generates an extended gradient in the cytoplasmic concentration of For3p (see Fig. S4F).
This, in agreement with the large rate of surface pressure increase (see Figure 5), suggests that BID-α6 binds efficiently to the lipid surface via electrostatic interactions, but without penetrating deep into the monolayer, as also suggested by the moderate πmax values.
This characteristic may explain the large rate of bacteremia caused by clone AbH12O-A2.
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