Sentence examples for the labelling procedure from inspiring English sources

Furthermore, labelling efficiency and characteristics of the labelling procedure and the labelled compound (e.g. concerning reaction speed and output) determine the utility of this concept for further applications in bioassays.

Once the labelling procedure has been established and the labelled products have been characterized they can be employed in further work.

In this case, regardless if fluorescence or elemental labelled antibodies are applied it is a prerequisite that the labelling procedure does not affect antibody functionality [21,31,44].

For implementation of this concept into routine analysis or diagnostics the applicability has to be verified in real life samples with different matrices, because this can significantly affect the recovery of the labelling procedure (e.g. unspecific interactions during labelling or separation of labelled and unlabelled compounds).

For the labelling procedure, 60 μg DOTA-NOC acetate was dissolved in 150 μl of 0.25 M sodium acetate buffer.

To increase the specific activity, we studied the effect of the buffer used during the labelling procedure: NaAc, NH4Ac, HEPES and MES buffer.

All the labelling procedures were carried out in the dark.

Tissue-sectioning (100 μm) was performed using the Leica VT1000S Vibratome (Leica, Heerbrugg, Switzerland) and the labelling procedures were previously described in detail elsewhere [ 18].

After stopping the labeling procedure, the Hy3-labeled samples were hybridized on the miRCURYTM LNA Array (v.18.0) (Exiqon) according to array manual.

After the labeling procedure was terminated, the Hy3-labeled samples were hybridized according to the instructions for the miRCURY LNA microRNA array.

The labeling procedure does not affect ATP binding, as the EC50 of ATP in channel activation was unaffected with labeled proteins.