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KM values were normalized to the KM of TetR.
In particular, the Km of the enzymes for both the substrates decreased significantly.
The Km of variant 108 decreased more than 2-fold, compared to that of the wild-type enzyme.
Using this assay, we determined the Km of two acceptor substrates, N-acetyl-l-lysine methyl ester and aminoacetonitrile.
The kcat of 2 was 3.4% of 1, but the Km of 2 was 10-fold that of 1.
The Michaelis Menten constant (Km) of NG was 26 μM, which is comparable to the Km of CoI (10 μM).
Decreasing the Km of this enzyme using a protein engineering approach would be a good target for improving isopropanol production and avoiding acetate accumulation in the culture medium.
While diabetes significantly decreased the brain specific activity (Vmax) of AChE soluble form by 42%, it caused a fivefold increase of the Km of the membrane-bound form.
Glucose exerts its effect on the PEPCK gene within 4 h, at physiologic concentrations, and with an EC50 of 6.5 mM, which approximates the Km of glucokinase.
The Km of endoglucanase from the strain Hahella chejuensis KCTC 2396 was 1.8 mg/ml (Ghatge et al. 2014).
By contrast, the Km of ChoKβ for ethanolamine was lower than ChoKα1.
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