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The isolates remaining in circulation are as virulent as those circulating during the prevaccine era (7 – 9 ).
The isolates remained within the respective genotypes when the P1 and P2 genome regions were used to construct the phylogenetic trees.
Sulphate reduction rates of all the isolates remained the same (0.0333 gL−1 das−100%s 100 % reductions were noticed in all the cases.
The isolate remained capnophilic after 5 subcultures.
The overall divergence value was nonetheless small (<1%); hence, the isolate remained within the NV cluster.
However, the isolate remained sensitive by repeat phage test suggesting phage failure to detect resistance.
The isolates remain susceptible only to vancomycin, linezolid, rifampin, and daptomycin – antibiotics that are not used for animal treatment.
The host tropisms and pathogenicity of the isolates remain to be clarified, and the relevance of these findings to public health and veterinary medicine deserves further investigation.
Of the 208 isolates, 158 (76%) clustered by IS 6110 alone; 100 (48%) of the isolates remained clustered even with the addition of spoligotyping data to further differentiate the TB strain.
The proportion of Enterobacter cloacae isolates remained constant (3.1% to 3.6%).
The ten isolates remaining (9.62 %) did not amplify successfully.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com